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The first is called chromosome (or primer) walking and starts with sequencing the first piece. The next (contiguous) piece of the sequence is then sequenced using a primer which is complementary to the end of the first sequence read and so on. This technique doesn't require much assembling, but you need a lot of primers and it is relatively ...
SOLiD applies sequencing by ligation and dual base encoding. The first SOLiD system was launched in 2007, generating reading lengths of 35bp and 3G data per run. After five upgrades, the 5500xl sequencing system was released in 2010, considerably increasing read length to 85bp, improving accuracy up to 99.99% and producing 30G per 7-day run. [10]
Whereas high sequence coverage for a genome may indicate the presence of repetitive sequences (and thus be masked), for a transcriptome, they may indicate abundance. In addition, unlike genome sequencing, transcriptome sequencing can be strand-specific, due to the possibility of both sense and antisense transcripts. Finally, it can be difficult ...
The New Year -- and the entire month of January -- is often the busiest time at the gym. This increase is largely driven by New Year's resolutions as more people set fitness and health goals. Read...
2. Chick-fil-A Chicken Noodle Soup. Price: $4.15 cup / $6.35 bowl Chick-fil-A’s chicken noodle soup is leagues better than it has any right to be. There’s so much flavor in this broth, and the ...
Extinguishers let you stay farther away while putting out a blaze, and they're a better choice for fires that aren't contained to a stovetop or oven, for instance. Most home cooks will encounter a ...
The method was first described by Elshire et al. (2011). [1] In summary, high molecular weight DNAs are extracted and digested using a specific RE previously defined by cutting frequently [7] in the major repetitive fraction of the genome. ApeKI is the most used RE. Barcode adapters are then ligated to sticky ends and PCR amplification is ...
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