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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.

  3. Use of DNA in forensic entomology - Wikipedia

    en.wikipedia.org/wiki/Use_of_DNA_in_forensic...

    To extract a blood meal from the abdomen of an insect to isolate and analyze DNA, the insect must first be killed by placing it in 96% ethanol. The killed insect can be stored at -20 °C until analysis. When it is time for analysis, the DNA must then be extracted by dissecting the posterior end of the abdomen and collecting 25 mg of tissue.

  4. Dische test - Wikipedia

    en.wikipedia.org/wiki/Dische_test

    When heated with DNA, it turns blue in the presence of DNA. A more intense blue color indicates a greater concentration of DNA. Mechanism The acid converts ...

  5. Forensic DNA analysis - Wikipedia

    en.wikipedia.org/wiki/Forensic_DNA_analysis

    Rapid DNA is a "swab in-profile out" technology that completely automates the entire DNA extraction, amplification, and analysis process. Rapid DNA instruments are able to go from a swab to a DNA profile in as little as 90 minutes and eliminates the need for trained scientists to perform the process.

  6. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    Nucleic acid extraction apparatus based on the Tajima pipette [14] [15] (see Fig. 2) are one of the most widespread instruments to perform the Boom method. [ 25 ] The Tajima pipette was invented by Hideji Tajima, [ 14 ] founder and president of Precision System Sciences (PSS) [ 25 ] Inc., a Japanese manufacturer of precision and measuring ...

  7. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.

  8. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    DNA sequencing; Expression cloning; Fluorescence in situ hybridization; Lab-on-a-chip; Comparison of nucleic acid simulation software; Northern blot; Nuclear run-on assay; Radioactivity in the life sciences; Southern blot; Differential centrifugation (sucrose gradient) Toeprinting assay; Several bioinformatics methods, as seen in list of RNA ...

  9. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    All genetic engineering processes involve the modification of DNA. Traditionally DNA was isolated from the cells of organisms. Later, genes came to be cloned from a DNA segment after the creation of a DNA library or artificially synthesised. Once isolated, additional genetic elements are added to the gene to allow it to be expressed in the host ...