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Transposon mutagenesis, or transposition mutagenesis, is a biological process that allows genes to be transferred to a host organism's chromosome, interrupting or modifying the function of an extant gene on the chromosome and causing mutation. [1] Transposon mutagenesis is much more effective than chemical mutagenesis, with a higher mutation ...
A bacterial DNA transposon. A transposable element (TE), also transposon, or jumping gene, is a type of mobile genetic element, a nucleic acid sequence in DNA that can change its position within a genome, sometimes creating or reversing mutations and altering the cell's genetic identity and genome size. [1] [2]
The discovery of transposons was made by Barbara McClintock who noticed that these elements could actually change the color of the maize plants she was studying, providing quick evidence of one outcome from transposon movement. [25] Another example is the Tol2 DNA transposon in medaka fish that is said to be the result of their variety in ...
When additional nucleotides are inserted near or into a locus, the locus can suffer a frameshift mutation that could prevent it from being properly expressed into polypeptide chain. Transposon-based Insertional inactivation is considered for medical research from suppression of antibiotic resistance in bacteria to the treatment of genetic ...
Avian leukosis virus is an example of a virus that causes disease by insertional mutagenesis. Newly hatched chicks infected with the Avian leukosis virus will begin to form tumours that will begin to appear in their bursa of Fabricius (like the human thymus). This viral gene insertion is also known as a promoter insertion as it drives the ...
Types of mutations that can be introduced by random, site-directed, combinatorial, or insertional mutagenesis. In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant genes, proteins, strains of bacteria, or other genetically modified organisms. The ...
Dr. Demetre Daskalakis, director of the National Center for Immunization and Respiratory Diseases, said the CDC is assessing a sample from that patient to determine if it has any concerning mutations.
One such mutation, L372P, is a mutation of amino acid 372 in the Tn5 transposase. This amino acid is generally a leucine residue in the middle of an alpha helix. When this leucine is replaced with a proline residue the alpha helix is broken, introducing a conformational change to the C-terminal domain, separating it from the N-terminal domain ...