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This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Steam at 134 °C (273 °F) can achieve a desired level of sterility in three minutes, while achieving the same level of sterility in hot air requires two hours at 160 °C (320 °F). [11] Methods of air removal include: Downward displacement (or gravity-type): As steam enters the chamber, it fills the upper areas first as it is less dense than air.
The proper time and temperature for dry heat sterilization is 160 °C (320 °F) for 2 hours or 170 °C (340 °F) for 1 hour, and in the case of High Velocity Hot Air sterilisers, 190°C (375°F) for 6 to 12 minutes. [3] Items should be dry before sterilization since water will interfere with the process.
Soil steam sterilization (soil steaming) is a farming technique that sterilizes soil with steam in open fields or greenhouses. Pests of plant cultures such as weeds, bacteria, fungi and viruses are killed through induced hot steam which causes vital cellular proteins to unfold. Biologically, the method is considered a partial disinfection.
An autoclave, which uses steam under pressure, is used in microbiology laboratories and similar environments for sterilization. Steam, especially dry (highly superheated) steam, may be used for antimicrobial cleaning even to the levels of sterilization. Steam is a non-toxic antimicrobial agent. [11] [12]
Infusion-based, where the liquid is pumped through a nozzle into a chamber with high-pressure steam at a relatively low concentration, providing a large surface contact area. This method achieves near-instantaneous heating and cooling and even distribution of temperature, avoiding local overheating.
There are multiple ways to clean your oven, and steam cleaning is one of them. Geared towards lighter messes, steam cleaning your oven can take a bit of work, but the results are well worth it.
Koch's or Arnold's steam sterilizer: used for steam sterilization A pressure cooker: used as a portable autoclave Biological and chemical indicators: Used to ascertain if a certain process has been completed, e.g. spores used in an autoclave are killed if autoclaving is properly done Filters: •Candle filter:
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