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A lysochrome is a soluble dye used for histochemical staining of lipids, which include triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV dissolve in the lipid and show up as colored regions. The dye does not stick to any other substrates, so a quantification or qualification of lipid presence can be obtained.
In the laboratory, Solvent Black 3 is used for Sudan staining. Similar dyes include Oil Red O, Sudan III, and Sudan IV. It can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids. It is used in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
Common name Synonyms C.I. generic name C.I. number Class CAS number Acid Black 52 Acid Black 52 15711 azo 5610-64-0: Acid Blue AS Weak Acid Blue Acid Blue 25: 62055 anthraquinone: 6408-78-2: Acid fuchsin: Acid Magenta Acid Rubin Acid Violet 19 42685 triarylmethane: 3244-88-0: Acid orange 3 Acid orange 3 10385 nitro 6373-74-6: Acid orange 19 ...
[2] [3] Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). [ 4 ]
Oil Red O is one of the dyes used for Sudan staining. Similar dyes include Sudan III, Sudan IV, and Sudan Black B. The staining has to be performed on fresh samples, as alcohol fixation removes most lipids. Oil Red O largely replaced Sudan III and Sudan IV, as it provides much deeper red color and the stains are therefore much easier to see.
Staining dyes are dyes used for staining in microbiology and histology. Subcategories. This category has the following 2 subcategories, out of 2 total. I.
Sudan stain test is often used to determine the level of fecal fat to diagnose steatorrhea. A small sample is dissolved in water or saline, glacial acetic acid is added to hydrolyze the insoluble salts of fatty acids , a few drops of alcoholic solution of Sudan III are added, the sample is spread on a microscopic slide, and heated twice to boil.
Sudan III is a dye used for Sudan staining. Similar dyes include Oil Red O, Sudan IV, and Sudan Black B. They are used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507(304) nm. [6]