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Tube cap color or type in order of draw Additive Usage and comments Blood culture bottle: Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms: Usually drawn first for minimal risk of contamination. [1] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms ...
The tubes have micronized silica particles which help clot the blood before centrifugation, and a gel at the bottom which separates whole blood cells from serum. [1] Silica nanoparticles induce coagulation through contact activation of coagulation factor XII (Hageman factor). [ 2 ]
A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing.
These specially designed bottles have long necks that fit into the evacuated tubes holders that are use for regular venipuncture collection. These bottles also allow for collection of other blood specimens via evacuated tubes, to be collected without additional venipuncture. [citation needed]
a clear solution of blood plasma in the upper phase (which can be separated into its own fractions, see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets in the middle, and; erythrocytes (red blood cells) at the bottom of the centrifuge tube.
The load in a laboratory centrifuge must be carefully balanced. This is achieved by using a combination of samples and balance tubes which all have the same weight or by using various balancing patterns without balance tubes. [2] It is an interesting mathematical problem to solve the balance pattern given n slots and k tubes with the same weight.
After centrifugation, one can distinguish a layer of clear fluid (the plasma), a layer of red fluid containing erythrocytes, and a thin layer in between.Composing less than 1% of the total volume of the blood sample, the buffy coat (so-called because it is usually buff in hue), contains most of the leukocytes and thrombocytes.
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