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  2. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  3. MIQE - Wikipedia

    en.wikipedia.org/wiki/MIQE

    It also acted as a précis for the broader form of the guidelines. [5] Other researchers have been creating further versions for specific forms of qPCR that may require a supplementary or different set of items to check, including single-cell qPCR [6] and digital PCR (dPCR). [7]

  4. Environmental Product Declaration - Wikipedia

    en.wikipedia.org/wiki/Environmental_Product...

    Criteria contained in a PCR includes: a description of the product category, the goal of the LCA, functional units, system boundaries, cut-off criteria, allocation rules, environmental impact categories, information on the product's use phase, required units, LCA calculation procedures, requirements for data quality assessment, and other ...

  5. Property condition assessment - Wikipedia

    en.wikipedia.org/wiki/Property_condition_assessment

    Property condition assessments (PCAs) (also known as the property condition report, or PCR) are due diligence projects associated with commercial real estate.Commercial property and building inspections are important for clients seeking to know the condition of a property or real estate they may be purchasing, leasing, financing or simply maintaining.

  6. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    RT-PCR. Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.

  7. Digital polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Digital_polymerase_chain...

    Droplet Digital PCR (ddPCR) is a method of dPCR in which a 20 microliter sample reaction including assay primers and either Taqman probes or an intercalating dye, is divided into ~20,000 nanoliter-sized oil droplets through a water-oil emulsion technique, thermocycled to endpoint in a 96-well PCR plate, and fluorescence amplitude read for all ...

  8. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.

  9. Multiplex polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Multiplex_polymerase_chain...

    Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform.

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