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[222] [223] CRISPR diversity is used as an analysis target to discern phylogeny and diversity in bacteria, such as in xanthomonads by Martins et al., 2019. [224]: 552 Early detections of plant pathogens by molecular typing of the pathogen's CRISPRs can be used in agriculture as demonstrated by Shen et al., 2020. [224]: 553
Land area used for genetically modified crops by country (1996–2009), in millions of hectares. In 2011, the land area used was 160 million hectares, or 1.6 million square kilometers. [54] In the US, by 2014, 94% of the planted area of soybeans, 96% of cotton and 93% of corn were genetically modified varieties.
CRISPR gene editing is a revolutionary technology that allows for precise, targeted modifications to the DNA of living organisms. Developed from a natural defense mechanism found in bacteria, CRISPR-Cas9 is the most commonly used system, that allows "cutting" of DNA at specific locations and either delete, modify, or insert genetic material.
The IGI sustainable agriculture program and its Plant Genomics and Transformation Facility has developed CRISPR protocols for editing over 30 common crop species, [17] and has worked on developing applications including protecting the world's chocolate supply from cacao swollen shoot virus, [55] [56] removing toxic cyanide precursors in cassava ...
Genetically modified crops (GM crops) are genetically modified plants that are used in agriculture. The first crops developed were used for animal or human food and provide resistance to certain pests, diseases, environmental conditions, spoilage or chemical treatments (e.g. resistance to a herbicide).
The first trial genetically modified food was a tomato engineered to have a longer shelf life (the Flavr Savr), which was on the market briefly beginning on May 21, 1994. [1] The first direct-consumption tomato was approved in Japan in 2021. [ 2 ]
It is far less effective at gene correction. Methods of base editing are under development in which a “nuclease-dead” Cas 9 endonuclease or a related enzyme is used for gene targeting while a linked deaminase enzyme makes a targeted base change in the DNA. [69] The most recent refinement of CRISPR-Cas9 is called Prime Editing.
Yield10 has also commenced development of CRISPR-enabled technology to impact crop yield. The company received a nonregulated status letter from the USDA-APHIS Biotechnology Regulatory Services (BRS) acknowledging that its camelina line has had a gene disrupted using CRISPR/Cas9 gene editing technology, resulting in the desired phenotype.
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