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RNA polymerase IV (RNAP IV) is an enzyme that synthesizes small interfering RNA (siRNA) in plants, which silence gene expression. [ 1 ] [ 2 ] [ 3 ] RNAP IV belongs to a family of enzymes that catalyze the process of transcription known as RNA Polymerases , which synthesize RNA from DNA templates. [ 4 ]
The transcription initiation at mutant MLP species was significantly reduced compared with that of the wild type or species in which there was a CAAT mutant. The failure to restore the normally functional adenoviruses, exhibited by a CAAT box, is consistent with the idea that the CAAT box plays a vital role in the adenovirus MLP and is ...
In most prokaryotes, a single RNA polymerase species transcribes all types of RNA. RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, forming the holoenzyme.
After the LECA, some 2 billion years ago, the eukaryotes diversified into a crown group, which gave rise to animals, plants, fungi, and protists. Eukaryogenesis , the process which created the eukaryotic cell and lineage, is a milestone in the evolution of life, since eukaryotes include all complex cells and almost all multicellular organisms.
Ribosomes are the macromolecular machines that are responsible for mRNA translation into proteins. The eukaryotic ribosome, also called the 80S ribosome, is made up of two subunits – the large 60S subunit (which contains the 25S [in plants] or 28S [in mammals], 5.8S, and 5S rRNA and 46 ribosomal proteins) and a small 40S subunit (which contains the 18S rRNA and 33 ribosomal proteins). [6]
RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. [11] It consists of RNA polymerase II, a subset of general transcription factors , and regulatory proteins known as SRB proteins.
In cases for sibling species, comparison of the rDNA segment including ITS tracts among species and phylogenetic analysis are made satisfactorily. [14] [15] The different coding regions of the rDNA repeats usually show distinct evolutionary rates. As a result, this DNA can provide phylogenetic information of species belonging to wide systematic ...
Eukaryotic organisms (animals, plants, fungi and protists) store most of their DNA inside the cell nucleus as nuclear DNA, and some in the mitochondria as mitochondrial DNA or in chloroplasts as chloroplast DNA. [5] In contrast, prokaryotes (bacteria and archaea) store their DNA only in the cytoplasm, in circular chromosomes.