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Brushes used to collect samples for cytology. Liquid-based cytology is a method of preparing samples for examination in cytopathology.The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid.
Hanks' salts is a collective group of salts rich in bicarbonate ions, formulated in 1940 by the microbiologist John H. Hanks. [1] Typically, they are used as a buffer system in cell culture media and aid in maintaining the optimum physiological pH (roughly 7.0–7.4) for cellular growth.
Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride. [ 1 ] [ 2 ] Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).
The third staining solution is composed of three dyes, Eosin Y, Light Green SF yellowish, and Bismarck brown Y in 95% ethyl alcohol with a small amount of phosphotungstic acid and lithium carbonate. [ 12 ] [ 2 ] This solution, designated EA, followed by a number that denotes the proportion of the dyes, other formulations include EA-36, EA-50 ...
Lugol's solution provides a strong source of free iodine and iodide to reef inhabitants and macroalgae. Although the solution is thought to be effective when used with stony corals, systems containing xenia and soft corals are assumed to be particularly benefited by the use of Lugol's solution. Used as a dip for stony and soft or leather corals ...
Alsever's solution is a saline liquid used to prevent coagulation of blood. [ 1 ] [ 2 ] It is composed of 2.05% dextrose , 0.8% sodium citrate , 0.055% citric acid , and 0.42% sodium chloride . For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution.
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared ...
Example of a cytocentrifuge. A cytocentrifuge, sometimes referred to as a cytospin, [1] is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined. [2]