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To prepare the sample for application, a small amount of blood (from a heel puncture, for example) or urine (from a diaper, for example) is applied to a piece of filter paper. Then a small disc is punched from the center of the spot of blood or urine, and the disc applied to the surface of a seeded, minimal-medium agar plate that contains added ...
EDTA is preferred for hematology tests because it does minimum damage to cell morphology. Sodium citrate is the anticoagulant used in specimens collected for coagulation tests. The majority of chemistry and immunology tests are performed on serum, which is produced by clotting and then separating the blood specimen via centrifuge.
In medicine, some blood tests are conducted on capillary blood obtained by fingerstick (or fingerprick) (or, for neonates, by an analogous heelprick). The site, free of surface arterial flow, where the blood is to be collected is sterilized with a topical germicide, and the skin pierced with a sterile lancet. [1]
Capillary bleeding: Capillary bleeding usually occurs in superficial wounds, such as abrasions. The colour of the blood may vary somewhat ( distal portion of circulation with oxygenated and unoxygenated blood mixing), and generally oozes in small amounts, as opposed to flowing or spurting.
A blood lancet, or simply lancet, is a small medical implement used for capillary blood sampling. A blood lancet, sometimes called a lance, is similar to a scalpel style lancet, but with a double-edged blade and a pointed end. It can even be a specialized type of sharp needle.
Arterial blood sampling, such as by radial artery puncture. This can be done for arterial blood gas analysis. Capillary blood sampling, generally by using a blood lancet for puncture, followed by sampling by capillary action with a test strip or small pipe. This is common for routine diabetic monitoring for glucose.
Phlebotomy is the process of making a puncture in a vein, usually in the arm, with a cannula for the purpose of drawing blood. [1] The procedure itself is known as a venipuncture , which is also used for intravenous therapy .
The distal end of the tibia is the preferred site because it is easy to access and the most reliable. Depending on the procedure, a variety of needles are used for IO. For example, "standard steel hypodermic, butterfly, spinal, trephine, sternal, and standard bone marrow needles are used." Needles that have a short shaft are preferred and safe.