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Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious ).
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Tryptic (trypticase) soy agar (TSA) is a general-purpose medium produced by enzymatic digestion of soybean meal and casein. It is frequently the base medium of other agar types; for example, blood agar plates are made by enriching TSA plates with blood.
Colonies of Micrococcus luteus on Tryptic Soy Agar. Cultivation 48 hours, 37°C. Trypticase soy agar or Tryptic soy agar (TSA) is a growth media for the culturing of moderately to non fastidious bacteria. It is a general-purpose, non-selective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
In microbiology laboratories, E. cloacae is frequently grown at 30 °C on nutrient agar or at 35 °C in tryptic soy broth. [1] It is a rod-shaped, Gram-negative bacterium, is facultatively anaerobic, and bears peritrichous flagella. It is oxidase-negative and catalase-positive. [2]
YPD medium bottle and YPD agar plate. YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete medium for yeast growth. It contains yeast extract, peptone, double-distilled water, and glucose (dextrose). [1]
Tryptic soy-serum-bacitracin-vancomycin (TSBV) is a type of agar plate medium used in microbiological testing to select for Aggregatibacter actinomycetemcomitans (A. a.). [1] It was described by Jørgen Slots in 1982, who also discovered the role of A.a. in periodontitis. [2] Per litre, TSBV contains: [3] 40 g tryptic soy agar; 1 g yeast extract
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.