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Nitrocellulose (also known as cellulose nitrate, flash paper, flash cotton, guncotton, pyroxylin and flash string, depending on form) is a highly flammable compound formed by nitrating cellulose through exposure to a mixture of nitric acid and sulfuric acid.
Filter the mixture through a filter disk made of nitrocellulose. Proteins bind to nitrocellulose, but DNA does not. Any DNA that is retained on the filter is there because it is interacting with the protein. Dry the filters and count. Measure the off-rate: Take the filter with DNA-protein complex bound on it; Wash the filter with buffer
A nitrocellulose slide is different from a nitrocellulose membrane, which usually filters protein from solution (i.e.: physician's office pregnancy tests), but that it serves a similar goal: to detect the presence and/or concentration level of certain biological material.
By 1959, Millipore made porous membrane filters of cellulose esters or other materials which resembled paper in sheet form, and were brittle when dry but friable when wet. [5] Filters consisted of nitrocellulose or polycarbonate membrane nucleopore filters ranging from pore size of 0.2 μm to 20 μm.
Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the following order from cathode to anode: sponge | three sheets of filter paper soaked in transfer buffer | gel | PVDF or nitrocellulose membrane | three sheets of filter paper soaked in transfer buffer | sponge.
Most depth filters used in pharmaceutical processes such as cell system harvesting are composed of cellulose fibres and filter aids. The direct flow design in depth filters provides a financially suitable solution by trapping the contaminants within the filter channel while ensuring the maximum recovery rate of the product.
In addition, compared to the nitrocellulose membrane which requires a high ionic strength buffer to bind the DNA fragments to the membrane, nylon charged membranes use buffers with very low ionic strength to transfer even small fragments of DNA of about 50 bp to the membrane, usually the DNA to be transferred is separated by polyacrylamide gel.
The cellulose fibers infiltration/filter aid applications can provide a protective layer to filter elements as powdered cellulose, besides promoting improved throughput and clarity. [citation needed] As ashless and non-abrasive filtration, make cleanup effortless after the filtering process without damage in pumps or valves. They effectively ...