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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
Pyrosequencing uses luciferase to generate light for detection of the individual nucleotides added to the nascent DNA, and the combined data are used to generate sequence reads. [81] This technology provides intermediate read length and price per base compared to Sanger sequencing on one end and Solexa and SOLiD on the other.
After reading the introduction I feel that the 454 should only be mentioned later under recent advantages. This way it appears that 454 is the only way pyrosequencing is applied. In addition, it is a bit of a repetition.CharonZ 10:06, 10 July 2007 (UTC)
A graph or chart or diagram is a diagrammatical illustration of a set of data. If the graph is uploaded as an image file, it can be placed within articles just like any other image. Graphs must be accurate and convey information efficiently. They should be viewable at different computer screen resolutions.
In a de Bruijn graph, there is a possibility of 4^k different nodes to make arrangements of a genome. The number of nodes used to create the graph can be reduced in number by considering only the k-mers found within the DNA strand of interest. Given sequence 1, it is possible to determine the nodes of size 7, or 7-mers, that will be in the graph.
Pyrosequencing has also been used to analyze bisulfite-treated DNA without using methylation-specific PCR. [7] [8] Following PCR amplification of the region of interest, pyrosequencing is used to determine the bisulfite-converted sequence of specific CpG sites in the region. The ratio of C-to-T at individual sites can be determined ...
In the mathematical field of graph theory, a word-representable graph is a graph that can be characterized by a word (or sequence) whose entries alternate in a prescribed way. In particular, if the vertex set of the graph is V , one should be able to choose a word w over the alphabet V such that letters a and b alternate in w if and only if the ...