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Field of view diameter in microscopy. In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes.
Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]
In wide-field multiphoton microscopy the high intensities are best achieved using an optically amplified pulsed laser source to attain a large field of view (~100 μm). [ 2 ] [ 3 ] [ 4 ] The image in this case is obtained as a single frame with a CCD without the need of scanning, making the technique particularly useful to visualize dynamic ...
A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
Dark-field microscopy produces an image with a dark background Operating principles of dark-field and phase-contrast microscopies Dark-field microscopy is a very simple yet effective technique and well suited for uses involving live and unstained biological samples, such as a smear from a tissue culture or individual, water-borne, single-celled ...
Apparent or Eye's field of view For telescopes, microscopes, or binoculars, the apparent field of view is a measure of the angular size of the image seen by the eye, through the eyepiece. In other words, it is how large the image appears (as distinct from the magnification).
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