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A microscope’s field of view is basically the diameter of that circular area that appears when you look into a microscope. Simple enough, right? We’ll look at some example and see how scientists and engineers calculate this and use this.
The field of view (FOV) on a microscope is the area that you can view through the eyepiece or camera when looking at a specimen. It is an essential parameter to consider when choosing a microscope to ensure that you can see your sample with enough detail and perspective.
Interactive Tutorial - Field of View Diameter. Exploring the effect of varying the field of view size on the viewable specimen area.
In a microscope, the microscopy field of view is the diameter of the viewing field measured at an intermediate plane of angle. To put it simply, it’s the diameter of the circular area you see when you look through the eyepiece of the microscope.
A microscope’s field of view (FOV) helps determine the approximate size of objects too small to measure with a ruler. To calculate the field of view diameter, divide the field number by the magnification number.
Field of view diameter in microscopy. In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes.
Field of View = FN ÷ (Objective Magnification x Auxiliary Lens Magnification) Typically the lower the magnification of the eyepiece, the higher the FN is. So for example, a 5x eyepiece might have a FN of 26mm, while a 10x eyepiece may be 22mm and a 30x eyepiece may only have a FN of 7mm.