Search results
Results from the WOW.Com Content Network
As its alternate name (5-methyluracil) suggests, thymine may be derived by methylation of uracil at the 5th carbon. In RNA, thymine is replaced with uracil in most cases. In DNA, thymine (T) binds to adenine (A) via two hydrogen bonds, thereby stabilizing the nucleic acid structures. Thymine combined with deoxyribose creates the nucleoside ...
Very short patch (VSP) repair is a DNA repair system that removes GT mismatches created by the deamination of 5-methylcytosine to thymine.This system exists because the glycosylases which normally target deaminated bases cannot target thymine (it being one of the regular four bases in DNA).
The 5'-hydroxyl group is protected by an acid-labile DMT (4,4'-dimethoxytrityl) group. Thymine and uracil, nucleic bases of thymidine and uridine, respectively, do not have exocyclic amino groups and hence do not require any protection.
As a result, specificity between 5-methylcytosine and 5-hydroxymethylcytosine is limited. [3] Another potential source of bias rises from polymerase chain reaction amplification of the library, which affects sequences with highly skewed base compositions due to high rates of polymerase sequence errors in high AT-content, bisulfite-converted DNA.
The RNA chain is synthesized from the 5' end to the 3' end as the 3'-hydroxyl group of the last ribonucleotide in the chain acts as a nucleophile and launches a hydrophilic attack on the 5'-triphosphate of the incoming ribonucleotide, releasing pyrophosphate as a by-[6] product. Due to the physical properties of the nucleotides, the backbone of ...
The first reaction is the simplest of the syntheses, by adding water to cytosine to produce uracil and ammonia: [2] C 4 H 5 N 3 O + H 2 O → C 4 H 4 N 2 O 2 + NH 3. The most common way to synthesize uracil is by the condensation of malic acid with urea in fuming sulfuric acid: [5] C 4 H 4 O 4 + NH 2 CONH 2 → C 4 H 4 N 2 O 2 + 2 H 2 O + CO
In enzymology, a methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (EC 2.1.1.74) is an enzyme that catalyzes the chemical reaction 5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH 2 ⇌ {\displaystyle \rightleftharpoons } tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
In enzymology, a tRNA (uracil-5-)-methyltransferase (EC 2.1.1.35) is an enzyme that catalyzes the chemical reaction S-adenosyl-L-methionine + tRNA containing uridine at position 54 ⇌ {\displaystyle \rightleftharpoons } S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54