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In the artificial environment of the aquarium, the nitrogen cycle effectively ends with the production of nitrates. In order that the nitrate level does not build up to a harmful level regular partial water changes are required to remove the nitrates and introduce new, uncontaminated water. [8]
The Berlin Method of biological filtration is a method for maintaining a clean and stable environment within a saltwater aquarium, typically a coral reef system. This method relies on the use of ample live rock (rock with live marine organisms and bacteria on or in it).
High levels of nitrite are also indicative of biofilter impending failure. Nitrate is the end-product of nitrification, and is the least toxic of the nitrogen compounds, with 96-hour exposure LC 50 values in freshwater in excess of 1,000 mg/L. [6]
Direct reduction from nitrate to ammonium, a process known as dissimilatory nitrate reduction to ammonium or DNRA, [6] is also possible for organisms that have the nrf-gene. [ 7 ] [ 8 ] This is less common than denitrification in most ecosystems as a means of nitrate reduction.
The processed ammonia is converted to nitrite, which is then processed to nitrate. Weekly water changes (25% of aquarium volume), while vacuuming debris from the bottom of the tank, can solve this problem of nitrate build-up, provided the tank is not overcrowded. Build-up of algae is largely related to light level and mineral imbalance.
Fishless cycling is a form of "maturing" an aquarium.The goal of the process is to establish a robust colony of nitrifiers, with the ammonia source provided to allow nitrifiers ('beneficial bacteria,' although nitrifiers can also be archaea) to grow and reproduce coming from non-fish sources, hence 'fishless.'
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[12] [13] Complete nitrification, the conversion of ammonia to nitrate in a single step known as comammox, has an energy yield (∆G°′) of −349 kJ mol −1 NH 3, while the energy yields for the ammonia-oxidation and nitrite-oxidation steps of the observed two-step reaction are −275 kJ mol −1 NH 3, and −74 kJ mol −1 NO 2 − ...
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