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Saturation mutagenesis is commonly achieved by site-directed mutagenesis PCR with a randomised codon in the primers (e.g. SeSaM) [2] or by artificial gene synthesis, with a mixture of synthesis nucleotides used at the codons to be randomised. [3] Different degenerate codons can be used to encode sets of amino acids. [1]
In OMZs oxygen concentration drops to levels <10 nM at the base of the oxycline and can remain anoxic for over 700 m depth. [7] This lack of oxygen can be reinforced or increased due to physical processes changing oxygen supply such as eddy-driven advection, [7] sluggish ventilation, [8] increases in ocean stratification, and increases in ocean temperature which reduces oxygen solubility.
Saturation of protein binding sites; Saturation of enzymes with a substrate; Saturation of a solute in a solution, as related to the solute's maximum solubility at equilibrium Supersaturation, where the concentration of a solute exceeds its maximum solubility at equilibrium
The lysocline is the depth in the ocean dependent upon the carbonate compensation depth (CCD), usually around 5 km, below which the rate of dissolution of calcite increases dramatically because of a pressure effect. While the lysocline is the upper bound of this transition zone of calcite saturation, the CCD is the lower bound of this zone. [1]
The "saturation system", "saturation complex" or "saturation spread" typically comprises either an underwater habitat or a surface complex which includesof a living chamber, transfer chamber and submersible decompression chamber, [45] which is commonly referred to in commercial diving and military diving as the diving bell, [46] personnel ...
At the time, Jacques Cousteau and Edwin A. Link were pursuing privately funded saturation diving projects to study long-term underwater living. Link's efforts resulted in the first underwater habitat, occupied by aquanaut Robert Sténuit in the Mediterranean Sea at a depth of 61 m (200 ft) for one day on September 6, 1962.
The lower the aragonite saturation level, the more difficult it is for the organisms to build and maintain their skeletons and shells. The map below shows changes in the aragonite saturation level of ocean surface waters between 1880 and 2012. [107] To pick one example, pteropods are a group of widely distributed swimming sea snails.
This series of reactions governs the pH levels in the ocean and also dictates the saturation state of seawater, indicating how saturated or unsaturated the seawater is with carbonate ions. Consequently, the saturation state significantly influences the balance between the dissolution and calcification processes in marine biogenic calcifiers.