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Hershey–Chase experiment (by Alfred Hershey and Martha Chase) uses bacteriophage to prove that DNA is the hereditary material (1952). Meselson–Stahl experiment proves that DNA replication is semiconservative (1958). Crick, Brenner et al. experiment using frameshift mutations to support the triplet nature of the genetic code (1961).
Examples include a smiley face and a coarse map of China and the Americas, along with many three-dimensional structures such as cubes. [8] There are several DNA properties that make the molecule an ideal building material for DNA origami. DNA strands have a natural tendency to bind to their complementary sequences through Watson–Crick base ...
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode ...
In biology, parts of the DNA double helix that need to separate easily, such as the TATAAT Pribnow box in some promoters, tend to have a high AT content, making the strands easier to pull apart. [29] In the laboratory, the strength of this interaction can be measured by finding the melting temperature T m necessary to break half of the hydrogen ...
She plans to give 10 kits away to families who will agree to videotape their children using the kits. Clow believes the videos will help market the kits so she can produce and sell more next year.
Insert the fragments of DNA into vectors that were cut with the same restriction enzyme. Use the enzyme DNA ligase to seal the DNA fragments into the vector. This creates a large pool of recombinant molecules. These recombinant molecules are taken up by a host bacterium by transformation, creating a DNA library. [9] [10]
Selected portions of the DNA nucleotide sequence are copied into a corresponding RNA nucleotide sequence, which either encodes a protein (if it is an mRNA) or forms a 'structural' RNA, such as a transfer RNA (tRNA) or ribosomal RNA (rRNA) molecule. Each region of the DNA helix that produces a functional RNA molecule constitutes a gene. [15]
Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
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