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Primary isolate is a pure microbial or viral sample that has been obtained from an infected individual, rather than grown in a laboratory. In chemistry and bacteriology, the verb isolate means to obtain a pure chemical, bacteriological or viral sample. The noun 'isolate' refers to the sample itself. [citation needed]
Another laboratory separation tool is the affinity magnetic separation (AMS), which is more suitable for the isolation of prokaryotic cells. [2] IMS deals with the isolation of cells, proteins, and nucleic acids through the specific capture of biomolecules through the attachment of small-magnetized particles, beads, containing antibodies and ...
Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
In microbiology, the term isolation refers to the separation of a strain from a natural, mixed population of living microbes, as present in the environment, for example in water or soil, or from living beings with skin flora, oral flora or gut flora, in order to identify the microbe(s) of interest. [1]
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
These primary cells can then be further expanded and transferred into fresh dishes through micropropagation. Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, [ 3 ] or ...
Besides the culture of well-established immortalised cell lines, cells from primary explants of a plethora of organisms can be cultured for a limited period of time before senescence occurs (see Hayflick's limit). Cultured primary cells have been extensively used in research, as is the case of fish keratocytes in cell migration studies. [88 ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]