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A Lindbergh perfusion pump, c. 1935, an early device for simulating natural perfusion. Perfusion is the passage of fluid through the circulatory system or lymphatic system to an organ or a tissue, [1] usually referring to the delivery of blood to a capillary bed in tissue. Perfusion may also refer to fixation via perfusion, used in histological ...
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The OFM probes are perfused with a physiological solution (the perfusate) which equilibrates with the ISF of the surrounding tissue. Operating flow rates range from 0.1 to 10 μL/min. OFM allows unrestricted exchange of compounds via an open structure across the open exchange area of the probe.
For example, in a system that incorporates syringe pumps, channels for perfusion inlet, perfusion outlet, waste, and cell loading would need to be added for the cell culture maintenance. [15] Perfusion in microfluidic cell culture is important to enable long culture periods on-chip and cell differentiation. [16]
Figure 5: Perfusion culture set-up is working on a laboratory table. A thermo plate maintains the desired temperature of 37°C. During culture a peristaltic pump transports the medium for example with 1.25 mL/h from a storage bottle (left side) to the waste bottle (right side).
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Ex vivo lung perfusion, EVLP, is a form of machine perfusion aimed at sustaining the active aerobic cellular metabolism of donor lungs outside the donor's body prior to lung transplantation. This medical preservation technique typically occurs within a specialised machine engineered to mimic the conditions of the natural circulatory system .
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