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DESeq2 employs the "size factor" normalization method, which adjusts for differences in sequencing depth between samples. [1] This normalization ensures that the expression values of genes are comparable across samples, allowing for accurate identification of differentially expressed genes.
Using DESeq2 as a framework, DEvis provides a wide variety of tools for data manipulation, visualization, and project management. DEXSeq is Bioconductor package that finds differential differential exon usage based on RNA-Seq exon counts between samples. DEXSeq employs negative binomial distribution, provides options to visualization and ...
When sequencing RNA other than mRNA, the library preparation is modified. The cellular RNA is selected based on the desired size range. For small RNA targets, such as miRNA, the RNA is isolated through size selection. This can be performed with a size exclusion gel, through size selection magnetic beads, or with a commercially developed kit.
The sample size is an important feature of any empirical study in which the goal is to make inferences about a population from a sample. In practice, the sample size used in a study is usually determined based on the cost, time, or convenience of collecting the data, and the need for it to offer sufficient statistical power. In complex studies ...
In bioinformatics, sequence analysis is the process of subjecting a DNA, RNA or peptide sequence to any of a wide range of analytical methods to understand its features, function, structure, or evolution.
4841 53610 Ensembl ENSG00000147140 ENSMUSG00000031311 UniProt Q15233 Q99K48 RefSeq (mRNA) NM_007363 NM_001145408 NM_001145409 NM_001145410 NM_001252518 NM_023144 RefSeq (protein) NP_001138880 NP_001138881 NP_001138882 NP_031389 NP_001239447 NP_075633 Location (UCSC) Chr X: 71.25 – 71.3 Mb Chr X: 100.47 – 100.49 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Non-POU domain ...
Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers . [ 3 ]
13629 Ensembl ENSG00000167658 ENSMUSG00000034994 UniProt P13639 P58252 RefSeq (mRNA) NM_001961 NM_007907 RefSeq (protein) NP_001952 NP_031933 Location (UCSC) Chr 19: 3.98 – 3.99 Mb Chr 10: 81.01 – 81.02 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic ...