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A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the rearrangement is present. Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique ...
Q-FISH. Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and ...
This 16th-century fish stall shows many traditional fish products. The term fish processing refers to the processes associated with fish and fish products between the time fish are caught or harvested, and the time the final product is delivered to the customer. Although the term refers specifically to fish, in practice it is extended to cover ...
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
Download as PDF; Printable version; ... (e.g. in fish) just below the epidermis, amongst other chromatophores. ... Many analytical procedures involve the use of a ...
The first step of nitrification in the biofilter consumes alkalinity and lowers the pH of the system. [10] Keeping the pH in a suitable range (5.0-9.0 for freshwater systems) is crucial to maintain the health of both the fish and biofilter. pH is typically controlled by the addition of alkalinity in the form of lime (CaCO 3) or sodium hydroxide ...
Bath treatment (fishkeeping) Bath treatments are easy to carry out disease treatments that originated since the earliest days of goldfish culture. One of the most effective procedure is called the salt bath which is quite effective in eradicating ciliated parasites from the fish. However, there are also useless, thus not recommended, bath ...
Four-stroke buccal pumping is used by some basal ray-finned fish and aquatic amphibians such as Xenopus and Amphiuma. [1] This method has several stages. These will be described for an animal starting with lungs in a deflated state: First, the glottis (opening to the lungs) is closed, and the nostrils are opened. The floor of the mouth is then ...