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  2. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    There are two basic types of optical microscopes: simple microscopes and compound microscopes. A simple microscope uses the optical power of a single lens or group of lenses for magnification. A compound microscope uses a system of lenses (one set enlarging the image produced by another) to achieve a much higher magnification of an object.

  3. Eyepiece - Wikipedia

    en.wikipedia.org/wiki/Eyepiece

    For a compound microscope the corresponding formula is ... thus from the expression given earlier for the angular magnification of a compound microscope

  4. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...

  5. Magnification - Wikipedia

    en.wikipedia.org/wiki/Magnification

    Without oil immersion, the maximum usable magnification is around 800×. For details, see limitations of optical microscopes. Small, cheap telescopes and microscopes are sometimes supplied with the eyepieces that give magnification far higher than is usable. The maximum relative to the minimum magnification of an optical system is known as zoom ...

  6. Confocal microscopy - Wikipedia

    en.wikipedia.org/wiki/Confocal_microscopy

    Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]

  7. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    Bright-field microscopes have low apparent optical resolution due to the blur of out-of-focus material; Bright-field microscopes typically produce low contrast with most biological samples, as few absorb light to a great extent. Samples that are naturally colorless and transparent cannot be seen well, e.g. many types of mammalian cells.

  8. Numerical aperture - Wikipedia

    en.wikipedia.org/wiki/Numerical_aperture

    "Microscope Objectives: Numerical Aperture and Resolution" by Mortimer Abramowitz and Michael W. Davidson, Molecular Expressions: Optical Microscopy Primer (website), Florida State University, April 22, 2004. "Basic Concepts and Formulas in Microscopy: Numerical Aperture" by Michael W. Davidson, Nikon MicroscopyU (website).

  9. Microscopic scale - Wikipedia

    en.wikipedia.org/wiki/Microscopic_scale

    Whilst compound microscopes were first developed in the 1590s, the significance of the microscopic scale was only truly established in the 1600s when Marcello Malphigi and Antonie van Leeuwenhoek microscopically observed frog lungs and microorganisms. As microbiology was established, the significance of making scientific observations at a ...

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