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In bioinformatics, BLAST (basic local alignment search tool) [3] is an algorithm and program for comparing primary biological sequence information, such as the amino-acid sequences of proteins or the nucleotides of DNA and/or RNA sequences.
A BLAST interface is provided to allow users to search for DNA or protein sequences against the Ensembl Genomes. It can be accessed by the header, located on top of all Ensembl Genome pages, titled BLAST. The BLAST search can be configured to search against individual species or collections of species (maximum of 25).
BLAT indexes the genome/protein database, retains the index in memory, and then scans the query sequence for matches. BLAST, on the other hand, builds an index of the query sequences and searches through the database for matches. [1] A BLAST variant called MegaBLAST indexes 4 databases to speed up alignments. [9]
Local search with fast k-tuple heuristic, slower but more sensitive than BLAST: Both: GGSEARCH, GLSEARCH Global:Global (GG), Global:Local (GL) alignment with statistics: Protein: Genome Magician Software for ultra fast local DNA sequence motif search and pairwise alignment for NGS data (FASTA, FASTQ). DNA: Hepperle D (www.sequentix.de) 2020 ...
HTML is the default output format for NCBI's web-page. Results for NCBI-BLAST are presented in graphical format with all the hits found, a table with sequence identifiers for the hits having scoring related data, along with the alignments for the sequence of interest and the hits received with analogous BLAST scores for these. [8]
The BLAST family of search methods provides a number of algorithms optimized for particular types of queries, such as searching for distantly related sequence matches. BLAST was developed to provide a faster alternative to FASTA without sacrificing much accuracy; like FASTA, BLAST uses a word search of length k , but evaluates only the most ...
The database search by BLAST requires input data to be in a correct format (e.g. FASTA, GenBank, PIR or EMBL format). Users may also designate the specific databases to be searched, select scoring matrices to be used and other parameters prior to the tool run.
Gene search - Search genes by name, symbol or synonym directly from the landing page; Gene nomenclature guidelines - Echinobase is the official body responsible for echinoderm gene naming; Genome browser with tracks for RNA-seq and ATAC-seq data - Echinobase uses JBrowse; BLAST - Users can BLAST against supported genomes, RNA, and protein sequences