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  2. Polarimeter - Wikipedia

    en.wikipedia.org/wiki/Polarimeter

    The ratio, the purity, and the concentration of two enantiomers can be measured via polarimetry. Enantiomers are characterized by their property to rotate the plane of linear polarized light. Therefore, those compounds are called optically active and their property is referred to as optical rotation. Light sources such as a light bulb, Tungsten ...

  3. Chiral analysis - Wikipedia

    en.wikipedia.org/wiki/Chiral_analysis

    Able to differentiate between enantiomers and from the racemate; (+) from (-) and (±) Spectroscopic: Polarimetry: Polarimetry uses the innate property of chiral molecules to rotate the plane-polarized light in equal and opposite direction. This method can be used to distinguish between enantiomers and from the racemate; (+) from (-) and (±)

  4. Specific rotation - Wikipedia

    en.wikipedia.org/wiki/Specific_rotation

    The CRC Handbook of Chemistry and Physics defines specific rotation as: For an optically active substance, defined by [α] θ λ = α/γl, where α is the angle through which plane polarized light is rotated by a solution of mass concentration γ and path length l.

  5. Optical rotation - Wikipedia

    en.wikipedia.org/wiki/Optical_rotation

    If two enantiomers are present in equal proportions, then their effects cancel out and no optical activity is observed; this is termed a racemic mixture. But when there is an enantiomeric excess, more of one enantiomer than the other, the cancellation is incomplete and optical activity is observed. Many naturally occurring molecules are present ...

  6. Mutarotation - Wikipedia

    en.wikipedia.org/wiki/Mutarotation

    The observed rotation of the sample is the weighted sum of the optical rotation of each anomer weighted by the amount of that anomer present. Therefore, one can use a polarimeter to measure the rotation of a sample and then calculate the ratio of the two anomers present from the enantiomeric excess, as long as one knows the rotation of each pure anomer.

  7. Polarimetry - Wikipedia

    en.wikipedia.org/wiki/Polarimetry

    Polarimetry is the measurement and interpretation of the polarization of transverse waves, most notably electromagnetic waves, such as radio or light waves. Typically polarimetry is done on electromagnetic waves that have traveled through or have been reflected , refracted or diffracted by some material in order to characterize that object.

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  9. Chiral derivatizing agent - Wikipedia

    en.wikipedia.org/wiki/Chiral_derivatizing_agent

    (R)-α-methoxy-α-(trifluoromethyl)- phenylacetic acid (Mosher's acid). In analytical chemistry, a chiral derivatizing agent (CDA), also known as a chiral resolving reagent, is a derivatization reagent that is a chiral auxiliary used to convert a mixture of enantiomers into diastereomers in order to analyze the quantities of each enantiomer present and determine the optical purity of a sample.