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Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D). [3]
A diauxic growth curve refers to the growth curve generated by an organism which has two growth peaks. The theory behind the diauxic growth curve stems from Jacques Monod's Ph.D. research in 1940. A simple example involves the bacterium Escherichia coli ( E. coli ), the best understood bacterium.
The method of enumeration [9] of surviving cells used by VCC is termed quantitative growth kinetics (QGK). It relates the kinetic time taken for the turbidity of a bacterial batch microbiological culture in a well of a 96-well microplate to reach a threshold difference in turbidity to a 10-fold dilution series of calibration growth curves.
μ is the growth rate of a considered microorganism, μ max is the maximum growth rate of this microorganism, [S] is the concentration of the limiting substrate S for growth, K s is the "half-velocity constant"—the value of [S] when μ/μ max = 0.5. μ max and K s are empirical (experimental) coefficients to the Monod equation. They will ...
Subculturing can also be used for growth curve calculations (ex. generation time) [2] and obtaining log-phase microorganisms for experiments (ex. Bacterial transformation). [3] Typically, subculture is from a culture of a certain volume into fresh growth medium of equal volume, this allows long-term maintenance of the cell line.
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One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.).