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Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot 'force' its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.
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When the Integration Host Factor was first discovered, it was only known for the site-specific recombination of bacteriophage. [4] This is all we knew for a while but through another article, we were able to find that with further research, IHF plays a key role in the scope of physiological processes of E. Coli, including site-specific recombination activities, phage packaging and partitioning ...
Phages like the lambda phage use their site specific recombinases to integrate their DNA into the host genome during lysogeny. P1 phage DNA on the other hand, exists as a plasmid in the host. The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are ...
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Lambda holin S (Lysis protein S of phage lambda, holin S105; TC# 1.E.2.1.1) is the prototype for class I holins. It has 3 TMSs with the N-terminus in the periplasm and the C-terminus in the cytoplasm. Its 107 codon sequence encodes two proteins with opposing functions, the holin, S105, and the holin inhibitor, S107.
Today's Wordle Answer for #1255 on Monday, November 25, 2024. Today's Wordle answer on Monday, November 25, 2024, is BROWN. How'd you do? Next: Catch up on other Wordle answers from this week.
There is an upper limit on the amount of DNA that can be packed into a phage (a maximum of 53 kb), therefore to allow foreign DNA to be inserted into phage DNA, phage cloning vectors may need to have some non-essential genes deleted, for example the genes for lysogeny since using phage λ as a cloning vector involves only the lytic cycle. [14]