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Concentrated nitric acid is added to a protein solution from the side of the test tube to form two layers. A white ring appears between the two layers if the test is positive. [1] Heller's test is commonly used to test for the presence of proteins in urine. [2] This test was discovered by the Austrian Chemist, Johann Florian Heller (1813-1871).
In 1773, Hilaire Rouelle obtained crystals containing urea from human urine by evaporating it and treating it with alcohol in successive filtrations. [64] This method was aided by Carl Wilhelm Scheele's discovery that urine treated by concentrated nitric acid precipitated crystals.
Uric acid is a heterocyclic compound of carbon, nitrogen, oxygen, and hydrogen with the formula C 5 H 4 N 4 O 3. It forms ions and salts known as urates and acid urates, such as ammonium acid urate. Uric acid is a product of the metabolic breakdown of purine nucleotides, and it is a normal component of urine. [1]
Gmelin's test is a chemical test used for detecting the presence of bile pigments in urine. It is named after Leopold Gmelin, who introduced the test. [1] [2] [3] Five millilitres of urine is slowly added to five millilitres of concentrated nitric acid in a test-tube. Different coloured rings between the two layers are visible if bile pigments ...
Urine contains water-soluble by-products of cellular metabolism that are rich in nitrogen and must be cleared from the bloodstream, such as urea, uric acid, and creatinine. A urinalysis can detect nitrogenous wastes of the mammalian body.
Urine pH varies with diet and a wide range of values occur in healthy subjects, although it is most commonly slightly acidic. Because the kidneys are involved in regulating acid-base balance, the urine is typically acidic in people with metabolic or respiratory acidosis and alkaline in those with alkalosis.
Murexide test is a color test for uric acid and some other purines. The (solid) sample is first treated with small volume of a concentrated acid such as hydrochloric acid, nitric acid, which is slowly evaporated away; subsequent addition of ammonia (NH 3) gives a purple color if uric acid was present, due to formation of murexide, or a yellow color that turns to red on heating if xanthine or ...
Maple syrup urine disease can be classified by its pattern of signs and symptoms or by its genetic cause. The most common and severe form of this disease is the classic type, which appears soon after birth, and as long as it remains untreated, gives rise to progressive and unremitting symptoms.