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Freezing is a common method of food preservation that slows both food decay and the growth of micro-organisms. Besides the effect of lower temperatures on reaction rates, freezing makes water less available for bacteria growth. Freezing is a widely used method of food preservation. Freezing generally preserves flavours, smell and nutritional ...
Freezing injury may occur at temperatures below 0 degrees Celsius. Symptoms of extracellular freezing include structural damage, dehydration, and necrosis. If intracellular freezing occurs, it will lead to death. Freezing injury is a result of lost permeability, plasmolysis, and post-thaw cell bursting.
Cryopreservation is an effective way to transport biological samples over long distances, store samples for prolonged periods of time, and create a bank of samples for users. Molecules, referred to as cryoprotective agents (CPAs), are added to reduce the osmotic shock and physical stresses cells undergo in the freezing process. [2]
Contact freezing uses physical contact other than air to transfer the heat. Direct contact freezing puts the product directly in contact with the refrigerant, while indirect contact freezing uses a plate in between. Plate freezing is the most common form of contact freezing. Food is put between cold metal plates and then lightly pressed to ...
To produce larger crystals, the product should be frozen slowly or can be cycled up and down in temperature in a process called annealing. The freezing phase is the most critical in the whole freeze-drying process, as the freezing method can impact the speed of reconstitution, duration of freeze-drying cycle, product stability, and appropriate ...
In physics and chemistry, flash freezing is the process whereby objects are rapidly frozen. [1] This is done by subjecting them to cryogenic temperatures , or it can be done through direct contact with liquid nitrogen at −196 °C (−320.8 °F).
Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for the electron microscopy and cryo-electron microscopy. [1]
A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods. Cryoprotectants are also used to ...