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  2. Bradford protein assay - Wikipedia

    en.wikipedia.org/wiki/Bradford_protein_assay

    The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.

  3. Protein methods - Wikipedia

    en.wikipedia.org/wiki/Protein_methods

    Bradford assay method uses a dye to bind to protein. Most commonly, Coomassie brilliant blue G-250 dye is used. When free of protein, the dye is red but once bound to protein it turns blue. [11] The dye-protein complex absorbs light maximally at the wavelength 595 nanometers and is sensitive for samples containing anywhere from 1 ug to 60 ug.

  4. Pierce Protein Assay - Wikipedia

    en.wikipedia.org/wiki/Pierce_Protein_Assay

    This method is able to detect as low as 25 μg/ml and up to 2000 μg/ml of protein in a 65 ul sample, using standard protocol. This method may be preferred for samples containing detergents or other reducing agents. This method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford ...

  5. Marion M. Bradford - Wikipedia

    en.wikipedia.org/wiki/Marion_M._Bradford

    Marion Mckinley Bradford (October 28, 1946 - May 3, 2021) was an American scientist [1] who developed and patented the Bradford protein assay, [2] a method to quickly quantify the amount of protein in a sample. [3] [4] His paper describing the method is among the most cited scholarly articles of all time. [5] [6] [7]

  6. Russia appears to be packing up its helicopters and air ... - AOL

    www.aol.com/russia-looks-packing-helicopters-air...

    In new satellite imagery, Russia's military appears to be packing up equipment at a key airbase in Syria. The images show transport aircraft ready to load cargo at the Hmeimim Air Base on Friday.

  7. Is Red Velvet Cake Actually Chocolate?

    www.aol.com/red-velvet-cake-actually-chocolate...

    Cocoa Powder Quantity: Red velvet cake recipes typically call for 2-3 tablespoons of cocoa powder, while a typical chocolate cake will call for ¾ cup to 1 cup of cocoa powder.

  8. "Hazardous drone operation" leads to two arrests in Boston - AOL

    www.aol.com/hazardous-drone-operation-leads-two...

    BOSTON – Two people were arrested Saturday night in Boston for what police describe as a "hazardous drone operation.". Robert Duffy, 42, of Charlestown, and 32-year-old Jeremy Folcik of ...

  9. Coomassie brilliant blue - Wikipedia

    en.wikipedia.org/wiki/Coomassie_brilliant_blue

    The Bradford assay uses the spectral properties of Coomassie brilliant blue G-250 to estimate the amount of protein in a solution. [19] A protein sample is added to a solution of the dye in phosphoric acid and ethanol. Under the acid conditions the dye is normally a brownish colour but on binding to the protein the blue form of the dye is produced.