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One example is capillary electrophoresis, [10] [12] in which electric fields are used to separate chemicals according to their electrophoretic mobility by applying an electric field to a narrow capillary, usually made of silica. In electrophoretic separations, the electroosmotic flow affects the elution time of the analytes.
The velocity of migration of an analyte in capillary electrophoresis will also depend upon the rate of electroosmotic flow (EOF) of the buffer solution. In a typical system, the electroosmotic flow is directed toward the negatively charged cathode so that the buffer flows through the capillary from the source vial to the destination vial.
Electroosmosis is the motion of liquid induced by an applied potential across a porous material, capillary tube, membrane or any other fluid conduit. Electroosmotic flow is caused by the Coulomb force induced by an electric field on net mobile electric charge in a solution.
Capillary electrochromatography (CEC) is an electrochromatography technique in which the liquid mobile phase is driven through a capillary containing the chromatographic stationary phase by electroosmosis. [3] [4] It is a combination of high-performance liquid chromatography and capillary electrophoresis. The capillaries is packed with HPLC ...
Capillary electrophoresis is a separation technique which uses high electric field to produce electroosmotic flow for separation of ions. Analytes migrate from one end of capillary to other based on their charge, viscosity and size. Higher the electric field, greater is the mobility.
Electroosmotic flow (or Electro-osmotic flow, often abbreviated EOF) is the motion of liquid induced by an applied potential across a capillary tube or microchannel. Electroosmotic flow is an essential component in chemical separation techniques, notably capillary electrophoresis.
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Electrophoresis is the basis for analytical techniques used in biochemistry for separating particles, molecules, or ions by size, charge, or binding affinity either freely or through a supportive medium using a one-directional flow of electrical charge. [10]