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Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue dots and curved linear structures (reticulum) in the cytoplasm. Supravital staining is a method of staining used in microscopy to examine living cells that
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Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).
NMB is a staining agent used in diagnostic cytopathology and histopathology, typically for staining immature red blood cells. It is a supravital stain. [2] It is closely related to methylene blue, an older stain in wide use.
In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain , the core concept being that the cell ...
In gastroenterology, esophageal pH monitoring is the current gold standard for diagnosis of gastroesophageal reflux disease (GERD). It provides direct physiologic measurement of acid in the esophagus and is the most objective method to document reflux disease, assess the severity of the disease and monitor the response of the disease to medical or surgical treatment.
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. [2] It is classified as an oxazine dye. N95 dust masks, eye shields, and gloves must all be worn when handling the chemical. [3]
The staining technique is named after the Russian physician Dmitri Leonidovich Romanowsky (1861–1921), who was one of the first to recognize its potential for use as a blood stain. [6] Stains that are related to or derived from the Romanowsky-type stains include Giemsa, Jenner, Wright, Field, May–Grünwald, Pappenheim and Leishman stains ...