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Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized. The test is also prepared on a slant to maximize bacterial growth for an even better indication of the use of citrate.
At this point, the bromothymol is already blue, and a few drops of BTB are used on a water slide. The specimen is mixed with blue BTB solution and fixed to a slide by a cover slip. It is sometimes used to determine cell walls or nuclei under the microscope. Bromothymol is used in obstetrics for detecting premature rupture of membranes. [11]
Use of citrate results in the creation of carbonates and bicarbonates as byproducts. Organisms degrading citrate must also use the ammonium salts, producing ammonia, [10] thus increasing the pH of the medium. [11] The increase in pH then causes color change in the bromothymol blue indicator, turning it blue.
Bacteria are inoculated on a medium containing sodium citrate and a pH indicator such as bromothymol blue. The medium also contains inorganic ammonium salts, which are utilized as sole source of nitrogen. Use of citrate involves the enzyme citrate lyase, which breaks down citrate to oxaloacetate and acetate.
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
Water weight, also known as water retention, is a buildup of excess water or fluid in the body's tissues, which can occur for a variety of reasons, Dr. Felice Schnoll-Sussman, gastroenterologist ...
Titration indicator table [11] Indicator name Indicator colour Transition interval (pH range) Color after high pH conditions Methyl Orange Orange/red 3.1 - 4.4 Yellow Methyl Red Red 4.4 - 6.3 Yellow Congo Red Blue 3.0 - 5.2 Red Phenolphthalein Colourless 8.3 - 10.0 Pink Thymolphthalein Colourless 9.3 - 10.5 Blue Bromophenol Blue Yellow 3.0 - 4.6
In a large bowl, stir cream cheese, hot sauce, blue cheese dressing, and 1/4 teaspoon salt until smooth and combined. Fold in 1 cup cheddar and 3 tablespoons blue cheese. Fold in chicken; season ...
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