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Clostridium beijerinckii; Clostridium tetanomorphum; Clostridium aurantibutyricum; These bacteria begin with butyrate fermentation, as described above, but, when the pH drops below 5, they switch into butanol and acetone production to prevent further lowering of the pH. Two molecules of butanol are formed for each molecule of acetone.
Acetone, butanol, and ethanol are the most common products of solventogenesis. Some species such as Clostridium beijerinckii, Clostridium puniceum and Clostridium roseum are able to further reduce acetone to isopropanol. Several species are able to produce additional solvents under various culture conditions.
Clostridium perfringens (gangrene, food poisoning) Clostridioides difficile (pseudomembranous colitis) Clostridium tetani ; Clostridium botulinum ; Clostridium acetobutylicum (acetone–butanol–ethanol fermentation, or ABE process) Clostridium haemolyticum; Clostridium novyi (gas gangrene, infectious necrotic hepatitis)
Clostridium saccharobutylicum is an indole and notably acetone, butanol and ethanol-producing bacterium, with type strain DSM 13864 T (= ATCC BAA-117 T). [ 1 ] [ 2 ] Its genome has been sequenced. [ 3 ]
The ABE fermentation via Clostridium beijerinckii or Clostridium acetobutylicum for instance is characterized by product inhibition. This means that there is a product concentration threshold that cannot be overcome, resulting in a product stream highly diluted in water. [11] Phase equilibrium diagram for 1-butanol–ethanol–water ternary mixture
Clostridium beijerinckii is a gram positive, rod shaped, motile bacterium of the genus Clostridium. It has been isolated from feces and soil. It has been isolated from feces and soil. Produces oval to subterminal spores.
Like ethanol, butanol can be produced by fermentation processes. Saccharomyces yeast are known to produce these higher alcohols at temperatures above 75 °F (24 °C). The bacterium Clostridium acetobutylicum can feed on cellulose (also an alcohol) to produce butanol on an industrial scale.
Glycolysis and fermentation of pyruvic acid by Clostridia yield the end products butyric acid, butanol, acetone, isopropanol, and carbon dioxide. [ 11 ] There is a commercially available polymerase chain reaction (PCR) test kit (Bactotype) for the detection of C. perfringens and other pathogenic bacteria.