Search results
Results from the WOW.Com Content Network
Intron-mediated enhancement (IME) is the ability of an intron sequence to enhance the expression of a gene containing that intron. In particular, the intron must be present in the transcribed region of the gene for enhancement to occur, differentiating IME from the action of typical transcriptional enhancers . [ 1 ]
During RNA splicing, U2 small nuclear RNA auxiliary factor 1 (U2AF35) and U2AF2 (U2AF65) interact with the branch site and the 3' splice site of the intron to form the lariat. It is thought that SR proteins that bind to ESEs promote exon splicing by increasing interactions with U2AF35 and U2AF65.
Some introns are known to enhance the expression of the gene that they are contained in by a process known as intron-mediated enhancement (IME). Actively transcribed regions of DNA frequently form R-loops that are vulnerable to DNA damage. In highly expressed yeast genes, introns inhibit R-loop formation and the occurrence of DNA damage. [60]
[7] [8] [9] This enhancer, located in the large intron, provided an explanation for the transcriptional activation of rearranged Vh gene promoters while unrearranged Vh promoters remained inactive. [10] Lately, enhancers have been shown to be involved in certain medical conditions, for example, myelosuppression. [11]
The word intron is derived from the terms intragenic region, [1] and intracistron, [2] that is, a segment of DNA that is located between two exons of a gene.The term intron refers to both the DNA sequence within a gene and the corresponding sequence in the unprocessed RNA transcript.
It is a process through which two or more exons from different genes can be brought together ectopically, or the same exon can be duplicated, to create a new exon-intron structure. [1] There are different mechanisms through which exon shuffling occurs: transposon mediated exon shuffling, crossover during sexual recombination of parental genomes ...
Splicing of group I introns is processed by two sequential transesterification reactions. [3] First an exogenous guanosine or guanosine nucleotide (exoG) docks onto the active G-binding site located in P7, and then its 3'-OH is aligned to attack the phosphodiester bond at the "upstream" (closer to the 5' end) splice site located in P1, resulting in a free 3'-OH group at the upstream exon and ...
This makes gene trapping more easily amenable for large scale projects than targeting. On the other hand, gene targeting can be used for genes with low transcriptions that would go undetected in a trap screen. The probability of trapping increases with intron size, while for gene targeting, small genes are just as easily altered.