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In cellular biology, stable cells are cells that multiply only when needed. They spend most of the time in the quiescent G 0 phase of the cell cycle but can be stimulated to enter the cell cycle when needed. Examples include the liver, the proximal tubules of the kidney and endocrine glands.
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro. The mutations required for immortality can occur ...
The 293T cell line was created in Michele Calos's lab at Stanford by stable transfection of the HEK 293 cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigen; it was originally referred to as 293/tsA1609neo. [9] The first reference to the cell line as "293T" may be its use to create the BOSC23 packaging ...
The Hayflick limit, or Hayflick phenomenon, is the number of times a normal somatic, differentiated human cell population will divide before cell division stops. [ 1 ] [ 2 ] The concept of the Hayflick limit was advanced by American anatomist Leonard Hayflick in 1961, [ 3 ] at the Wistar Institute in Philadelphia , Pennsylvania.
When viewing this in relation to the cell survival curve, separate cells are then plated parallel with increasing doses of radiation. Surviving fraction is the ratio between the number of colonies that survive said doses of radiation divided by the cells seeded when taking plating efficiency into account. [5]
MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted white male fetus. [ 1 ] [ 2 ] The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 ...
In the Margolus neighborhood, the lattice is divided into 2-cell blocks (or 2 × 2 squares in two dimensions, or 2 × 2 × 2 cubes in three dimensions, etc.) which are shifted by one cell (along each dimension) on alternate timesteps. [1] [2] [3]
The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. [2]In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to ...
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