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The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group.A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours.
Indole test positive: appearance of pink layer at top (e.g. Escherichia coli) Like many biochemical tests on bacteria, results of an indole test are indicated by a change in color following a reaction with an added reagent. Pure bacterial culture must be grown in sterile tryptophan or peptone broth for 24–48 hours before performing the test.
Proteus is a genus of Gram-negative bacteria. It is a rod shaped, aerobic and motile bacteria, which is able to migrate across surfaces due its “swarming” characteristic in temperatures between 20 and 37 °C. [1] Their size generally ranges from 0.4 to 0.8 μm in diameter and 1.0–3.0 μm in length. They tend to have an ammonia smell. [2]
The species C. amalonaticus, C. koseri, and C. freundii can use citrate as a sole carbon source. Citrobacter species are differentiated by their ability to convert tryptophan to indole (C. koseri is the only citrobacter to be commonly indole-positive), ferment lactose (C. koseri is a lactose fermentor), and use malonate.
P. hauseri is ONPG negative and PDA positive [citation needed]. Unlike the more commonly seen species of Proteus, P. hauseri is also able to convert tryptophan into indole, resulting in a positive indole test. P. hauseri shares a similar biochemical profile with Proteus vulgaris but can be differentiated by its ability to produce acid from ...
Below is a list of differential techniques and results that can help to identify Bacillus licheniformis from other bacteria and Bacillus species. [27] Anaerobic Growth: Positive; Voges Proskauer test: Positive; Acid produced from D-glucose: Positive; L-arabinose: Positive; D-mannitol: Positive; Starch hydrolysis: Positive; Nitrate reduction ...
Proteus vulgaris is a rod-shaped, nitrate-reducing, indole-positive and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water, and fecal matter.
The Ehrlich reagent is similar to a number of other indole tests: The van Urk reagent, which uses 0.125 g of p-DMAB, 0.2 mL of ferric chloride solution (25 mg/mL) in 100 mL of 65% sulfuric acid. [9] [10] [11] This is sometimes referred to as the Hofmann reagent or p-DMAB-TS (Test Solution) and gives slightly different colours with different ...