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Turbidimetric inhibition immuno assay (TINIA) is a type of immunoassay that uses turbidimetry as the measurement principle and is used for many commercial immunoassays, e.g. measurement of HbA1c%, [1] Digoxin etc. in whole blood sample in several commercial assays employ this principle.
Turbidimetry (the name being derived from turbidity) is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it. Light is passed through a filter creating a light of known wavelength which is then passed through a cuvette containing a solution.
RF is tested by collecting blood in a plain tube (5 mL is often enough). The serum is tested for the presence of RF. There are different methods available, which include nephelometry, turbidimetry, agglutination of gamma globulin-coated latex particles or erythrocytes.
Turbidity is the cloudiness or haziness of a fluid caused by large numbers of individual particles that are generally invisible to the naked eye, similar to smoke in air.The measurement of turbidity is a key test of both water clarity and water quality.
Illustration of hook effect adapted from Schiettecatte et al. [1] The hook effect refers to the prozone phenomenon, also known as antibody excess, or the postzone phenomenon, also known as antigen excess.
Diagnosis of health disorders such as blood cancers; Measuring genome size; A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.
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In whole blood (g/cm 3) In plasma or serum (g/cm 3) Water: Solvent 0.81-0.86 0.93-0.95 Acetoacetate: Produced in liver 8-40 × 10 −7: 4-43 × 10 −7: Acetone: product of bodyfat breakdown 3-20 × 10 −6: Acetylcholine: Neurotransmitter of the parasympathetic nervous system: 6.6-8.2 × 10 −8: Adenosine triphosphate: Energy storage total 3. ...