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In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrasts with positive staining, in which the actual specimen is stained.
Unlike negative staining, positive staining uses basic dyes to color the specimen against a bright background. While chromophore is used for both negative and positive staining alike, the type of chromophore used in this technique is a positively charged ion instead of a negative one. The negatively charged cell wall of many microorganisms ...
The diastase negative slide will show a magenta staining where glycogen is present within a section of tissue. The slide that has been treated with diastase will lack any positive PAS staining in those locations on the slide PAS staining is also used for staining cellulose. One example would be looking for implanted medical devices composed of ...
Unicolor plaque disclosing tablet Tricolor plaque disclosing gel—before and after. Disclosing tablets are chewable tablets that make dental plaque visible.. The tablets, sold over the counter in many countries, contain a dye (typically a vegetable dye, such as Phloxine B) that stains the plaque a bright color (typically red or blue).
Examples include iron (black stain), iodine (black), copper (green), nickel (green) and cadmium (yellow-brown). [3] Antibiotics. Tetracycline and its derivatives are capable of intrinsic discoloration (discussed below). However other antibiotics may form insoluble complexes with calcium, iron and other elements that cause extrinsic staining. [11]
PAS diastase stain is also used to identify alpha-1 antitrypsin globules in hepatocytes, which is a characteristic finding of alpha-1 antitrypsin deficiency. [2] PAS diastase stain is also used in diagnosing Whipple’s disease , as the foamy macrophages that infiltrate the lamina propria of the small intestine in this disease possess PAS ...
Rubber cups, also called prophy cups, are used in the hand-piece. Polishing paste, prophylactic paste, usually containing fluoride, is used with the rubber cups for polishing. Rubber cups should not be used over the cementum area as it may remove a layer of cementum at the cervical area. There are two popular types of prophy cups: 4 webs and 6 ...
Tooth whitening or tooth bleaching is the process of lightening the colour of human teeth. [1] Whitening is often desirable when teeth become yellowed over time for a number of reasons, and can be achieved by changing the intrinsic or extrinsic colour of the tooth enamel. [2]