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1969: Molecular hybridization of radioactive DNA to the DNA of cytological preparation by Pardue, M. L. and Gall, J. G. 1970: Restriction enzymes were discovered in studies of a bacterium, Haemophilus influenzae, by Hamilton O. Smith and Daniel Nathans, enabling scientists to cut and paste DNA. [44]
Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a particular chromosome. [4]In the 1960s, researchers Joseph Gall and Mary Lou Pardue found that molecular hybridization could be used to identify the position of DNA sequences in situ (i.e., in their natural positions within a chromosome).
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
MAGIChips, also known as "microarrays of gel-immobilized compounds on a chip" or "three-dimensional DNA microarrays", are devices for molecular hybridization produced by immobilizing oligonucleotides, DNA, enzymes, antibodies, and other compounds on a photopolymerized micromatrix of polyacrylamide gel pads of 100x100x20 μm or smaller size.
During the early 1960s, sophisticated genetic analysis of mutations in the lac operon of E. coli and in the rII locus of bacteriophage T4 were instrumental in defining the nature of both messenger RNA and the genetic code. The short-lived nature of bacterial RNAs, together with the highly complex nature of the cellular mRNA population, made the ...
Pardue was born in Lexington, Kentucky on September 15, 1933. [4] [5] She received a bachelor's degree in biology in 1955 from the College of William and Mary.Pardue received a master's degree in radiation biology in 1959 from the University of Tennessee, where she had been eligible for a Ph.D. but convinced the department to give her the master's degree instead, later explaining in an ...
James Herbert Taylor (January 14, 1916 in Corsicana, Texas – December 29, 1998 in Tallahassee, Florida) [1] [2] was an American molecular biologist and geneticist known for his research on chromosome structure and reproduction, which helped establish standards for the subsequent field of molecular genetics.
In chemistry, isovalent or second order hybridization is an extension of orbital hybridization, the mixing of atomic orbitals into hybrid orbitals which can form chemical bonds, to include fractional numbers of atomic orbitals of each type (s, p, d). It allows for a quantitative depiction of bond formation when the molecular geometry deviates ...