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In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern.
Frozen tissue cores with 2 mm diameter from the regions of interest are removed from frozen tissue OCT blocks at different freezing temperature since each tissue type has their own temperature preference at frozen stage. Then all the frozen tissue cores are inserted in a recipient OCT frozen block in a precisely spaced, array pattern.
Animal glue in granules. Animal glue is an adhesive that is created by prolonged boiling of animal connective tissue in a process called rendering. [1] In addition to being used as an adhesive, it is used for coating and sizing, in decorative composition ornaments, and as a clarifying agent.
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
Soft tissue and bone strengthen with exercise and weaken if it is not actively used. [11] [12] Horses that undergo long-term stall rest require a slow, progressive rehabilitation program to try to prevent re-injury of the original tissue or injury to a new area now weakened by prolonged disuse. In some cases of soft tissue injury, low-impact ...
The laser microtome has the ability to slice almost every tissue in its native state. Depending on the material being processed, slice thicknesses of 10 to 100 μm are feasible. Sectioning intervals can be classified mainly into either: Serial sectioning: obtaining a continuous ribbon of sections from a paraffin block and using all for slides.
Horses with the acute form of colic usually have a duration of colic less than 24 hours long, while chronic cases have mild but intermittent colic. Horses with the chronic form tend to have better prognosis. Rectal examination reveals a mass at the base of the cecum in 50% of cases. [19]
Orthopedic causes of lameness are very common and may be the result of damage to the hoof, bone, joints, or soft tissue. Horses are predisposed to orthopedic lameness by conformational flaws, poor hoof balance, working on poor footing, repetitive movements, poor conditioning for a given activity, and competing at a very high athletic level. [2]
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