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There is a single type strain for each prokaryotic species, but different culture collections may designate a unique name for the same strain. For example, the type strain of E. coli (originally strain U5/41) is called ATCC 11775 by the American Type Culture Collection, DSM 30083 by the German Collection of Microorganisms and Cell Cultures, JCM ...
The coding region of a gene, also known as the coding DNA sequence (CDS), is the portion of a gene's DNA or RNA that codes for a protein. [1] Studying the length, composition, regulation, splicing, structures, and functions of coding regions compared to non-coding regions over different species and time periods can provide a significant amount of important information regarding gene ...
By convention, the coding strand is the strand used when displaying a DNA sequence. It is presented in the 5' to 3' direction. Wherever a gene exists on a DNA molecule, one strand is the coding strand (or sense strand), and the other is the noncoding strand (also called the antisense strand, [3] anticoding strand, template strand or transcribed ...
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode ...
For example, in a typical gene a start codon (5′-ATG-3′) is a DNA sequence within the sense strand. Transcription begins at an upstream site (relative to the sense strand), and as it proceeds through the region it copies the 3′-TAC-5′ from the template strand to produce 5′-AUG-3′ within a messenger RNA (mRNA).
The former passed the strain to Ann Kuttner ("the Bact. coli obtained from Dr. Bordet") [18] and in turn to Eugène Wollman (B. coli Bordet), [19] whose son deposited it in 1963 (CIP 63.70) as "strain BAM" (B American), while André Gratia passed the strain to Martha Wollstein, a researcher at Rockefeller, who refers to the strain as "Brussels ...
The quickest way to identify to match an isolated strain to a species or genus today is done by amplifying its 16S gene with universal primers and sequence the 1.4kb amplicon and submit it to a specialised web-based identification database, namely either Ribosomal Database Project Archived 19 August 2020 at the Wayback Machine, which align the ...
For example, whole-genome sequencing of numerous isolates has revealed the single MLST lineage ST258 of Klebsiella pneumoniae comprises two distinct genetic clades, [16] providing additional information about the evolution and spread of these multi-drug resistant organisms, and disproving the previous hypothesis of a single clonal origin for ST258.