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Sectioning intervals can be classified mainly into either: Serial sectioning: obtaining a continuous ribbon of sections from a paraffin block and using all for slides. Step sections: collected at specified depths in the block.
Sections from this block are cut using a cryostat, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each frozen tissue array block can be cut into 100–500 sections, which can be subjected to independent tests.
These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Sections from this block are cut using a microtome, mounted on a microscope slide and then analyzed by any method of standard histological analysis. Each microarray block can be cut into 100 – 500 sections, which can be subjected to ...
The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome. [2]
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
Microtome can be used in sectioning of sufficiently thin slices. If the objects cannot satisfy the requirement of thickness, materials are required to be dehydrated using alcohol before section. [12] Three commonly used sectioning method are freehand section technique, paraffin method, and celloidin method.
Before selecting an area of the specimen block to be ultra-thin sectioned, the technician examines semithin or "thick" sections range from 0.5 to 2 μm. These thick sections are also known as survey sections and are viewed under a light microscope to determine whether the right area of the specimen is in a position for thin sectioning. "Ultra ...
Disposable plastic molds or embedding molds (Leukart's L blocks) for tissue paraffin block making w.r.t. Histopathology: used to make blocks of tissue for cutting into thin slices for microscopy Block holders (in histopathology) used to hold the tissue blocks during cutting •Refrigerated microtome