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In photophosphorylation, light energy is used to pump protons across a biological membrane, mediated by flow of electrons through an electron transport chain. This stores energy in a proton gradient. As the protons flow back through an enzyme called ATP synthase, ATP is generated from ADP and inorganic
The mobile electron carriers are, as usual, a lipid-soluble quinone and a water-soluble cytochrome. The resulting proton gradient is used to make ATP. In noncyclic electron transfer, electrons are removed from an excited chlorophyll molecule and used to reduce NAD + to NADH. The electrons removed from P840 must be replaced.
The chlorophyll molecule ultimately regains the electron it lost when a water molecule is split in a process called photolysis, which releases oxygen. The overall equation for the light-dependent reactions under the conditions of non-cyclic electron flow in green plants is: [27]
to NADPH or are used in non-cyclic electron flow. [1] DCMU is a chemical often used in laboratory settings to inhibit photosynthesis. When present, DCMU inhibits electron flow from photosystem II to plastoquinone.
Cyclic electron transport or cyclic photophosphorylation produces only ATP. The noncyclic variety involves the participation of both photosystems, while the cyclic electron flow is dependent on only photosystem I. Photosystem I uses light energy to reduce NADP + to NADPH + H +, and is active in both noncyclic and cyclic electron transport. In ...
Similar to the electron transport chain, the light-dependent reactions of photosynthesis pump protons into the thylakoid lumen of chloroplasts to drive the synthesis of ATP. The proton gradient can be generated through either noncyclic or cyclic photophosphorylation.
The electron transport chain of green sulfur bacteria—such as is present in the model organism Chlorobaculum tepidum—uses the reaction center bacteriochlorophyll pair, P840. When light is absorbed by the reaction center, P840 enters an excited state with a large negative reduction potential, and so readily donates the electron to ...
It is possible to introduce an artificial electron acceptor into the light reaction, such as a dye that changes color when it is reduced. These are known as Hill reagents. These dyes permitted the finding of electron transport chains during photosynthesis. Dichlorophenolindophenol (DCPIP), an example of these dyes, is widely used by experimenters.