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Many methods are used to identify cell lines, including isoenzyme analysis, human lymphocyte antigen (HLA) typing, chromosomal analysis, karyotyping, morphology and STR analysis. [35] One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA.
As the final product of iPSC reprogramming was similar in morphology, proliferation, gene expression, pluripotency, and telomerase activity, genetic and morphological markers were used as a way to determine what phase of reprogramming was occurring. [32] Reprogramming is defined into three phase: initiation, maturation, and stabilization. [33]
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This likely allows TET1 to demethylate an adjacent methylated cytosine. When human mammary epithelial cells (MCF-10A) were treated with H 2 O 2, 8-OHdG increased in DNA by 3.5-fold and this caused about 80% demethylation of the 5-methylcytosines in the MCF-10A genome. [25]
The phospholipids may range in chain length to branching. Ultimately, the phospholipid will determine the membrane properties, such as fluidity and charge, that will regulate the interactions with nearby proteins. In addition, the membrane oversees the development of the cell's morphology and cell sizes. [13]
To figure out how S phase entry can be affected by sporadic pulses of ERK activity at low EGF concentrations, they used MCF-10A cells co-expressing EKAR-EV and RFP-geminin and identified the pulses of ERK activity with the scoring and then align this ERK activity profiles with time of GFP-geminin induction.
The decoction of aerial parts of F. leucopyrus shows cytotoxic effects on breast cancer cells, particularly Her2 negative cells (MCF-7 and MDA-MB-231), compared to Her2 positive (SKBR-3) and non-cancerous cells (MCF-10A). This supports its traditional use for its anticancer properties.
Chicken cystatin quickly passed the membrane of MCF-10A neo T cells and inhibited cathepsin B when it was acylated with fatty acyl residues of 6-18 carbon atoms. [7] ...