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Optical density (OD) is directly proportional to the biomass in the cell suspension in a given range that is specific to the cell type. Using spectrophotometry for measuring the turbidity of cultures is known as turbidometry. This has made spectrophotometry the methods of choice for measurements of bacterial growth and related applications.
As we have already seen, opacity is not easily measured with standard photographic equipment—but the logarithm of opacity is continually measured since, in fact, it is the unit of image saturation known as density. Since density is a logarithm we must take the ratio of the anti-logarithms of the maximum and minimum densities in the image in ...
m is the optical mass or airmass factor, a term approximately equal (for small and moderate values of θ) to , where θ is the observed object's zenith angle (the angle measured from the direction perpendicular to the Earth's surface at the observation site).
The size of the eyes in mammals is relatively small; in humans, eye weight is 1% of the mass of the head, while in a starling it reaches 15%. Nocturnal animals (for example, tarsiers ) and animals that live in open landscapes have larger eyes.
Mass extinction coefficient, how strongly a substance absorbs light at a given wavelength, per mass density; Molar extinction coefficient, how strongly a substance absorbs light at a given wavelength, per molar concentration; Optical extinction coefficient, the imaginary part of the complex index of refraction
Spectrophotometer for OD600 and Cell Density Measurements. OD600 (Also written as O.D. 600, D 600, o.d. 600, OD 600) is an abbreviation indicating the optical density of a sample measured at a wavelength of 600 nm in 1 cm light path (unless otherwise stated).
Kleiber's plot comparing body size to metabolic rate for a variety of species. [1]Kleiber's law, named after Max Kleiber for his biology work in the early 1930s, states, after many observations that, for a vast number of animals, an animal's Basal Metabolic Rate scales to the 3 ⁄ 4 power of the animal's mass.
In this way the inverse function has many uses. For instance, certain ELISA assays have a standard curve whose concentrations can be fit extremely well to their optical density by a Gompertz function. Once the standards are thus fit to a Gompertz function, calculating the unknown concentration of samples in the assay from their measured optical ...