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  2. Cold Spring Harbor Protocols - Wikipedia

    en.wikipedia.org/wiki/Cold_Spring_Harbor_Protocols

    Cold Spring Harbor Protocols (formerly CSH Protocols) is an online scientific journal and methods database for biologists, published by Cold Spring Harbor Laboratory Press. Protocols are presented step-by-step and edited in the style that has made Molecular Cloning, Antibodies, Cells and many other CSH manuals essential [ tone ] to the work of ...

  3. Thermostable DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/Thermostable_DNA_Polymerase

    Molecular weight 80kDa [1] 94kDa [1] 94kDa [1] ... Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press; 3rd edition ...

  4. Tom Maniatis - Wikipedia

    en.wikipedia.org/wiki/Tom_Maniatis

    While an assistant professor of Biochemistry and Molecular Biology at Harvard, and a member of the Cold Spring Harbor Laboratory (CSHL) faculty, Maniatis collaborated with Drs. Fotis Kafatos and Argiris Efstratiadis to develop a method for synthesizing and cloning full length double stranded DNA copies of messenger RNA (termed “copy” or cDNA).

  5. Molecular cloning - Wikipedia

    en.wikipedia.org/wiki/Molecular_cloning

    Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...

  6. Recombinant DNA - Wikipedia

    en.wikipedia.org/wiki/Recombinant_DNA

    There are two fundamental differences between the methods. One is that molecular cloning involves replication of the DNA within a living cell, while PCR replicates DNA in the test tube, free of living cells. The other difference is that cloning involves cutting and pasting DNA sequences, while PCR amplifies by copying an existing sequence.

  7. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    It is a molecular method used, among other things, to recognize and count particular bacterial groupings. [1] ... Molecular Cloning (4th ed.). Cold Spring Harbor, ...

  8. Joseph Sambrook - Wikipedia

    en.wikipedia.org/wiki/Joseph_Sambrook

    Sambrook is best known for his studies on DNA tumor viruses and the molecular biology of normal and neoplastic cells.His Tumour Virus Group at Cold Spring Harbor identified and mapped all of the major genes of adenoviruses and SV40, determined their transcriptional control in infected and transformed cells, and elucidated the mechanism of integration of these viruses into the genome of the ...

  9. Cold Spring Harbor Laboratory - Wikipedia

    en.wikipedia.org/wiki/Cold_Spring_Harbor_Laboratory

    Cold Spring Harbor Laboratory (CSHL) is a private, non-profit institution with research programs focusing on cancer, neuroscience, plant biology, genomics, and quantitative biology. [2] It is located in Laurel Hollow on Long Island , New York.