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Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
In recent years, the confocal Laser Scanning Microscope (LSM) has become widely established as a research instrument. The present brochure aims at giving a scientifically sound survey of the special nature of image formation in a confocal LSM.
Confocal laser scanning microscopy (CLSM) is a type of fluorescence microscopy. Laser light induces fluorescence from samples to produce a 2D micrograph. This fluorescence is separated from incident light using a pinhole.
The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging appli-cations at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today’s sophisticated instruments are outlined.
The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today's sophisticated instruments are outlined.
Confocal laser scanning microscope - set up: The system is composed of a a regular ßorescence microscope and the confocal part, including scan head, laser optics, computer.
Confocal Laser Scanning Microscopy is a technique that allows researchers to focus a laser beam to a specific depth within a tissue to analyze the concentration of components at that level, enabling the creation of concentration profiles, particularly useful in drug product studies.
Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers.
The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today’s sophisticated instruments are outlined.
Laser Scanning Confocal Microscopy. Confocal microscopy offers several advantages over conventional optical microscopy, including controllable depth of field, the elimination of image degrading out-of-focus information, and the ability to collect serial optical sections from thick specimens.